The University Health Centre (PKU) was established to provide healthcare services exclusively to the university community, including students, staff, and eligible dependants.

PKU was initially known as the University Clinic, which began operations in 2006 at Tan Sri Aishah Ghani Residential College, Wang Ulu.

In 2009, the University Clinic was upgraded to the University Health Centre (PKU) and subsequently operated across Pauh Putra, Wang Ulu, and Sungai Chuchuh campuses.

As of 26 August 2024, all healthcare services provided by the University Health Centre are fully centralised at the new PKU building in Pauh Putra Campus.